SensiMedia Microbiological Test Methodology
Stand alone Kit
SensiMedia microbiological test methodology is based on the CO2 detection sensor technology.
It was developed to detect even single live microorganism without using any equipment for achieving the following objectives.
- Easy-to-use !
- Single live microorganism in a sample can be detected.
- More contaminated, Faster to be detected.
- Performance and repeatability of detection kit is rliable.
- Stand alone Test Kit ! (No Equipment required)
- An equipment is for automating detection and making a real time detection possible.
- The pH of a sample does not affect the detection.
- Color of a sample does not affect the detection.
- Kit should be enclosed type so that it is safe to detect live harmful microbes.
SensiMedia microbiological test methodology was invented by the company’s founder, Dr. Hiroyuki Ogawa, Ph.D. SensiMedia is only microbial test kit for faster detection of microbial contamination without using equipment. Biomatic VDCS is provided for fully automating detection.
Principle of Operation (CO2 sensor)
One of traditional microbial detection methods is to detect CO2 gas produced by growing microorganisms while incubating sample added liquid culure media.
SensiMedia is a microbiological test kit that is a sterilized test tube containing liquid culture media and a CO2 sensor inside. The sensor catches CO2 produced by microbe and when the catched amount of CO2 reaches to a certain amount, the sensor turns the color from blue to yellow. The timing coincides with microbe logarithmic growth stage and color change occurs rather quickly within one hour or so. Considering incubation time span as 24hr, the state change can be treated as digital response.
Microbial growth time measurement by Biomatic VDCS
The sensor of SensiMedia is blue at first. When it detects the presence of microbe, the sensor turns into yellow. Althogh the sensor itself does the detection, Biomatic VDCS incubates SensiMedia while monitoring the sensor of SensiMedia every 5 ｍｉｎｕｔｅｓ to decide whether the kit detected microbe or not, measuring a time from the begning of test untill the time microbe is detected.
Logarithmic scale graph of microorganism growth time
Prepare SensiMedia series inoculated by 1ml each from E. coli dilution series. Then measure the detection time for each diluted sample. Draw the regression line for each detection time on a logarithmic scale graph as shown below. This is one of methods to obtain the degree of microbial growthi in graphical form, for an example. The graph is normalized by obtaining the real microbial quqntity in the same sample.
More number of microbe in a SensiMedia, ealier the detection time.
Related Page
- Liquid media Performance:
Evaluation method of liquid culture media - Precise evaluation of liquid culture media is achieved by SensiMedia with microbial growth.
- Microbial
property characterization - Using standard culture media base such as SCD, property of microbe is characterized by changing parameters such as temperature, pH, nutrient composition and as such.