Anaerobe detect valication can be acheived by using Clostridium sporogenes as the standard species.

Anaerobe detection is validated by Clostridium sporogenes as standard species.

Clostridium sporogenes is detected automatically !

microbiological test system, MicroBio μ3D

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Set anaerobic plates and start a test !


Anaerobe detection is validated by Cl. sporogenes as standard species.

BioBall30 [Clostridium sporogenes ] was used as the standard species for validation of anaerobic detection method. The 31 colonies were detected for the standard.

Test System

MicroBio µ3D AutoScanner

Species strain

BioBall30
[ Clostridium sporogenes (NCTC 12935 )]

Agar Media

Thioglycollate ager pour plate

Sample

pour Plate in anaerobic conditioning bag

Incubation

35ºC, 42h

Detection Result

31 colonies were detected at 36h.
(one extra live cell was detected for BioBall30 standard.)

 

42hr

Plate Image

Count Marker

 

The above graph with plate image can be displayed during a test. The automatic detection was completed at 36hr for this example, detecting 31 colonies.

 

 

The same test was perform, using SCD pour plate. The followings are the test results. 

Test System

MicroBio µ3D AutoScanner

Species strain

BioBall30
[ Clostridium sporogenes (NCTC 12935 )]

Agar Media

SCD agar pour plate

Sample

pour Plate in anaerobic conditioning bag

Incubation

35ºC, 42h

Detection Result

Only 3 colonies were detected after 42h incubation.
(27 live cells were not detected.)

 

42hr

Plate Image

Count Marker

 

 

The above graph with plate image can be displayed during a test. Three colonies of the standard species were detected, yeilding 10%. Therefore, the use of SCD pour plated should be avoided for anaerobe detect validation.

 

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