Colony count range is wide！ (from 1 to few thousands.)
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Colony count range
Test samples are dilution series of ten times, containing different numbers of E. coli cells. The cell density was adjusted to 5, 50, 500, and 5000 cells/ml, respectively, and 1 ml of each test sample was applied to a plate. The results of TSIA are depicted in the above figure. Number of colonies counted by TSIA (cfu/plate) was 4, 51, 482 and 5342. In every plate, colonies were detected around 10-12hr and the counted number of colonies became a steady value around 13-15hr, reaching at the plateau on the graph.
It becomes increasingly difficult to verify the right colony count visually for higher range so that the colonies were counted by using special counter program with the saved plate image data step by step manually, going back in chlonological order, to verify the TSIA provided count.
Related Page
- [Colony Count Graph]
When two species are detected in a test sample, the colony count becomes staircase. - When two species are detected in a test sample, the colony detection and count graph of ＭｉｃｒｏＢｉｏ µ3D becomes staircase.
- [Rapid Detection and Count]
They are the results that show how MicroBio µ3D system detect and counts micro-colonies rapidly and automatically. - These are the performance results of automated rapid detection and count of MicroBio µ3D on E. coli, Aspergillus brasiliensis and Candida albicans. Detection is in real time.
- [Colony Count Unit]
A cluster of touched colonies is counted correctly as separated colonies. - CFU (Colony Forming Unit) has been used for counting microbial colonies as the unit. Using MicroBio µ3D system, the exact number can be used for counting colonies.
- [Invisible colonies detected]
Colonies under a piece of hamburger is detected. - MicroBio µ3D detects invisible colonies under a piece ot hamburger. The colonies become visible after a prolonged incubation, coming out from the rim of a meat piece.
- [Pricise Colony Count]
There are 4 colonies detected among a few hundred of hamburger fragments. - There are 4 E. coli colonies detected and counted precisely among a few hundred hamburger fragments in a poured cliform detection agar plate.