Reliable Coliform Negative Test is performed rapidly !
Add sample and incubate it !
When a coliform negative test is performed, nothing happens. Using SensiMedia, "negative" is determined rapidly since SensiMedia has good repeatability. If Biomatic VDCS is used for SensiMedia, test can be fully aoutomated.
SensiMedia SM is to test 1ml of sample. Add a sample to it and incubate it. When the presence of live microorganisms are detected, the color of sensor turns into yellow from blue. If sensor had been blue until the end of test protocol, then the test was negative.
Add sample and incubate to test !
The graph bwelow shows the detection performance of different kit for E. coli detection. Any detection kit inhibit growth of Bacillus during the test protocol. In case of BGLB, inhibitation is strongest among kits so that the growth of E. coli becomes slower, compared with liquid media that other kit uses.
* The graph is normalized by exact colony count provided by MicroBio μ3D.
Coliform Detection Kit
Catalog No. |
Product Name |
10^3 colonies Detection Time |
1 Microorganism Detection Time |
|---|---|---|---|
| Coliform detection | 7.5h |
9.5h |
|
| Coliform detection | 8.5h |
11.0h |
|
SM031 |
EC broth | 10.5h |
13.5h |
| BGLB | 12.5h |
15.5h |
- After the time passed the detection time for 1 microorganism, test is Negative and finished.
- If the test protocol is set to 24hr, then any above test kit can be used for coliform negative test.
As other kit, Lactose broth SensiMedia (SM018) and EE broth SensiMedia (SM028) are available. Choose any of them depending upon the purpose of detection.
SensiMedia SML is to test 5ml of sample. Since the amount of sample increases, coliform negative test becomes 5 times more secure, compared with the case SensiMedia SM is use.
SML016 Coliform detection （5ml sample）
The performance is as same as that of SM016.
SML016-20 Coliform detection （20ml sample)
The performance of SML016-20 is as same as that of SM016, but it can take 20ml of sample. Milk sample, for example, it performes as follows.
|
Before in use |
after 20ml of milk added Negative |
after 20ml of milk added Positive |
|---|---|---|
|
|
|
SML002 Coliform detection
The performance is as same as that of SM002.
SML002P Coliform detection kit for sample contains oil or fat (butter or margarine)
Specific amounts of lecithin and polysorbate are added to the liquid media so that fatty sample such as butter or margarine is mexed well with media.
Automated Coliform Negative Test
Qualitative Analysis
Using SM or SML type of SensiMedia, negative test can be automated by Boimatic VDCS.
Related Page
- Virus and macrophage plaques are quantifed accurately.
- Virus infected cells form plaques, and macrophage preyed bacteria form plaques. The plaque assay is a standard biological assay that counts and quantifies this plaque. MicrroBio μ3D collects data on the process of plaque formation during culture, allowing accurate plaque counting.
- [Rapid Coliform Detection]
Coliform is detected with a pour plate of 2ml milk-sample automatically ! - Traditional pour-plate method is used for the detection of coloform in seemingly impossible 2ml of milk-sample. Coliform can be detected faster in real time automatically by MicroBio μ3D AutoScanner.
- [Rapid Total Viable Count]
MicroBio µ3D automatically and rapidly enumerates Total Viable Count (TVC) of microorganisms on 100 plates being incubated at the same time. - MicroBio µ3D AutoScanner automatically, rapidly and precisely enumerates Total Viable Count (TVC) of microorganisms on 100 plates at the same time while the Standard Plate Count media plates are being incubated. The example is the case of cut vegetable mix sample.
- [Detect coliform and E. coli]
The presence of E. coli is checked after coliform is detected. - The presence of E. coli is checked by applying UV light to the SensiMedia for letting blue fluorescence emission after coliform is detected.
- [Membrane Filter Method]
Membrane filter method is fully automated as a rapid microbiological test method.
Each plate can be conditioned to anaerobic. - Membrane filter method is fully automated as a rapid microbiological test method. Each agar media plate with a filter can also be conditioned to anaerobic atmosphere.
- [Automated Mold Detection]
Mold colonies are detected and counted rapidly and full automatically. - Mold (fungi) and yeast colonies are detected and enumerated rapidly and automatically by MicroBio µ3D AutoScanner. The MicroBio PDA performs well for mold culturing.
- [Alicyclobacillus Detection]
Rapid Alicyclobacillus detection made easy at high temperature and low pH. - A rapid detection kit is available. (e.g.) Alicyclobacilluｓ in a sugar syrup sample can be screened for Alicyclobacillus and then checked for guaiacol production to confirm the presence of live A. acidoterrestris.
- [Salmonella Detection]
Rapid salmonella detection is easy for liquid, powder or solid sample. - Rapid salmonella detection is easy for liquid, powder or solid sample. SensiMedia takes any form of sample: shape, color, form.
- [Exophiala Detection]
Exophiala, black mold, is detected reliably, rapidly and automatically. - Exophiala is detected rapidly and automatically by MicroBio µ3D microbiological test system with MicroBio PDA.
- [Auto-Anaerobe Detection]
Anaerobes are detected faster and automatically, including anaerobes grow at high temperature. - Anaerobes such as Lactobacillus or Clostridium are detected rapidly and automatically by MicorBio µ3D microbiological test system.
- [Auto-Salmonella Detection]
Salmonella detection can be accelerated and automated. - Salmonella is detected rapidly and automatically by using MicroBio μ3D system with MicroBio Salmonella agar media, PPM001.
- [Aerobe (mold) Detection]
Aerobes such as mold can be detected faster and easily. - With SensiMedia aerobic detection kit, aerobes such as mold canbe detected faster and easily.
- [Auto-Clostridium Detection]
Anaerobe detection is validated by Clostridium sporogenes as standard species. - Clostridium in anaerobic atmosphere is detected rapidly and automatically by MicroBio µ3D microbiological test system.
- [Microbial Property Characterization]
It is an example of characterization performed on cocoa antimicrobial property. - For it is said that cocoa has antibacterial property, characterization was perfermed to find out how effective the property is, using a microbial growth graph.
- [Food poisoning microbe]
There are various rapid detection kits for food poisoning microbes ! - SensiMedia is microbial detection kit to test samples and is available to detect food poisoning microbes: Salmonella, Staphylococcus aureus, O157, Bacillus cereus, Listeria.