Exophiala is detected rapidly and automatically by MicroBio μ3D microbiological test system with MicroBio PDA.

     

 

 

Just set the PDA agar medium plate and start.

（We recommend MicroBio PDA.）

 

 

Among molds and fungi, Exophiala is said to be detected hard and is the troublesome mold that contaminates such product as beverages and daily necessities, causing quality problem.

 

Potato dextrose agar (PDA) medium is commonly used in the industry as the main medium for mold detection, but exophiala rarely grows on PDAs, so normal PDA has difficulty to detect exophiala. This is one of the main causes of the problem.

 

Sabouraud dextrose agar medium or MicroBio PDA can be used for reliable detection.
If you use this medium for inspection of the exophiala contamination with MicroBio μ3D AutoScanner that is a fully automated microbial detection system, you can realize how easy it is to detect this troublesome mold. You can say the same for membrane filter method.

The following is the detection test data of MicroBio μ3D AutoScanner for thedetection of exophilia on agar medium of PDA, GPLP, and Sabouraud dextrose.
As a result, it was confirmed that Sabouraud dextrose medium supports thegrowth of Exophilia better than PDA medium.
We are conducting comparative studies using graphs, but it is said that it isbetter to have a large number of samples in order to obtain a standarddistribution (bell shape), so that the sample should have a bacterial count of1,000 cells / ml or more.

 

The same sample of Exophiala .jeanselmei was added to each medium. However,as a result of culturing, PDA detected no colony.

 

 

 

PDA (Potato Dextrose) Agar media

Composition (　PDA39.0g / 　 water 1litter　)	Potato infusion(4.0g), Dextrose(20.0g), Agar(15.0g)
Species strain	Exophiala .jeanselmei(NBRC6858)
Sample	about1000-1500 cells / ml

　　　　　※With PDA, Exophiala detection becomes false negative.

 

 

 

 

 

GPLP （Glucose Peptone Agar with Lecithin & Polysorbate） Agar media

Composition ( / water 1litter　)	Yeast extract （2.0g）, Casein　Peptone（5.0g）, Gulcose（20.0g）, Agar （15.0g）, Magnesium sulfate（0.5g）,　Potassium dihydrogen phosphate（1.0g）, lecithin（15.0g）,  Polysorbate（807g）
Species strain	Exophiala .jeanselmei(NBRC6858)
Sample	about1000-1500 cells / ml

 

 

First image　 　Last image

 

 

 

Sabouraud Dextrose Agar media

Composition ( / water 1litter　)	Animal tissue-Pepsin digestion peptone（5.0g）,　　　　　　　　casein-Sui digestion peptone（5.0g）,Gulcose（40.0g）, agar（15.0g
Species strain	Exophiala .jeanselmei(NBRC6858)
Sample	about1000-1500 cells / ml

 

 

　 

First image　 　 Last image

 

 

 

MicroBio PDA

Composition ( / water 1litter　)	PDA modified
Species strain	Exophiala .jeanselmei(NBRC6858)
Sample	about1000-1500 cells / ml

 

 

　 

First image　 　 Last image

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